Southampton dumped holders Arsenal out of the FA Cup on Saturday, winning 1-0 courtesy of a Gabriel own goal to set up a fifth-round meeting with Wolves.
It was the first goal Arsenal had conceded in 508 minutes as they suffered the first loss in seven matches.
The much-changed Gunners, who have won the competition a record 14 times, fell behind midway through the first half against their energetic opponents.
Arsenal were without forward Pierre-Emerick Aubameyang and midfielder Emile Smith Rowe, while another in-form player, Bukayo Saka, was only introduced in the second half.
Mikel Arteta’s Gunners side were tepid as an attacking force during the first 45 minutes but looked more of a threat after the break.
However, Eddie Nketiah’s deflected shot in the 67th minute, which was superbly dealt with by home goalkeeper Fraser Forster, was their only effort of note.
Mikel Arteta, who won the FA Cup in August in his first season as Arsenal boss, said he was “really sad” to exit the competition.
“We had issues in the first half and unforced errors as a result of the high-press they do,” he told BT Sport. “In the second half we had two or three big chances but not enough.”
Southampton have eliminated the holders from the competition for the first time since beating Tottenham in a replay in 1902 while losing their previous seven FA Cup fixtures against the holders.
This is the third time in the past four seasons that Arsenal have failed to make it to the fifth round of the FA Cup.
This was Gunners’ boss Arteta’s first defeat in the FA Cup as a player or manager in 15 games (W13 D1), since featuring in Arsenal’s 1-0 loss to Blackburn in February 2013.
Southampton will face another Premier League side, Wolves, in the next round.