The captains of the 20 clubs in Primera División are to meet on Wednesday at the headquarters of the Spanish Association of Footballers to discuss their “concern” over a decision to stage a game in the US.
La Liga agreed a 15-year deal with US media company Relevent on Friday.
The Spanish players’ union, AFE, says players were not consulted by La Liga.
“Footballers once again feel marginalized in decision-making,” read an AFE statement.
“We believe their opinions are essential for the growth of Spanish football.
“We demand balance and common sense. If the greatness of football is the passion it generates, why are decisions made against it?”
Captains or representatives from each of the 20 teams in La Liga will attend the meeting at the AFE headquarters in Madrid.
Asked when the US-based match would take place and whether it would be a regular fixture, La Liga told BBC Sport last week it “can not confirm the teams, match, date or season”.
The AFE said players “do not understand the unilateral decisions”, adding: “La Liga is distancing the fans of the players, something that harms the show and the essence of football.”
Also on the agenda at the meeting is the kick-off times imposed on Primera clubs by the LFP, another unilateral bone of contention.
The players’ union will express its concerns about staging games on Friday and Monday, which has proved unpopular with fans and places demands on a small number of clubs (those involved in Europe are exempt), and about scheduling, with games occasionally starting on one day and finishing on another, as was the case with Real Madrid-Getafe on Sunday.