World number one Angelique Kerber was knocked out of the Italian Open in a surprise straight-set defeat by Estonia’s Anett Kontaveit on Wednesday.
The world number 68 won 6-4 6-0 in 56 minutes, meaning both Kerber and men’s top seed Andy Murray exited the Rome tournament at the second-round stage.
British number two Kyle Edmund also suffered a straight-set defeat by Argentina’s Juan Martin del Potro.
The 22-year-old world number 53 lost 7-5 6-4 in one hour and 46 minutes.
Fellow Briton Aljaz Bedene was also knocked out in the second round by world number two Novak Djokovic.
Del Potro, the 2009 US Open champion, will face Japan’s Kei Nishikori in the last 16.