Romania’s world number five Simona Halep and Canada’s world number seven Milos Raonic will be missing the Rio Olympics Games in Brazil because of concerns about the Zika virus.
Raonic, 25, was beaten in Sunday’s Wimbledon final by Andy Murray.
“I am making this decision for a variety of health concerns including the uncertainty around Zika,” he said.
Losing Wimbledon quarter-finalist Halep, 24, said: “I truly regret these circumstances.”
More than 20 male players have withdrawn from the golf competition at Rio 2016, with many concerned about the mosquito-borne virus.
Halep and Raonic are the first tennis players to make themselves unavailable because of Zika, which is linked to brain deformities in newborn babies.
“Family is much too important for me and I can’t risk not being able to have one of my own after my career in tennis is over,” said Halep.
Raonic, meanwhile, wrote on Facebook: “I do not wish to impact the decision of any other athlete heading to the Games.
“I look forward to cheering on Team Canada this summer.”
The United States’ John Isner has pulled out to focus on his preparations for tour events, while Nick Kyrgios will not be involved because of a dispute with the Australian Olympic Committee (AOC).