Champions Enyimba fell to their second straight loss in the Nigeria league after they were beaten 2-1 by hosts Nasarawa United on Sunday.
A win would have catapulted Enyimba to the top of the Nigeria league table.
Enyimba, who lost 3-1 at Wikki Tourists last week to end a nine-match unbeaten run, took the lead at Nasarawa through Ifeanyi George after seven minutes.
However, the home team drew level from the penalty spot courtesy of Esosa Igbinoba on 24 minutes.
And four minutes later, Abdularahman Bashir scored what proved to be the match-winner from close range.